A dual wavelength-activatable gold nanorod complex for synergistic cancer treatment.

A dual wavelength-activatable gold nanorod complex for synergistic cancer treatment.

Nanoscale (2015-07-01)

Dennis B Pacardo, Bhanu Neupane, S Michaela Rikard, Yue Lu, Ran Mo, Sumeet R Mishra, Joseph B Tracy, Gufeng Wang, Frances S Ligler, Zhen Gu

PMID26122945

ABSTRACT

A multifunctional gold nanorod (AuNR) complex is described with potential utility for theranostic anticancer treatment. The AuNR was functionalized with cyclodextrin for encapsulation of doxorubicin, with folic acid for targeting, and with a photo-responsive dextran-azo compound for intracellular controlled drug release. The interaction of a AuNR complex with HeLa cells was facilitated via a folic acid targeting ligand as displayed in the dark-field images of cells. Enhanced anticancer efficacy was demonstrated through the synergistic combination of promoted drug release upon ultraviolet (UV) light irradiation and photothermal therapy upon infrared (IR) irradiation. This multifunctional AuNR-based system represents a novel theranostic strategy for spatiotemporal delivery of anticancer therapeutics.

MATERIALS

Product Number

Brand

Product Description

71320

Sigma-Aldrich

Sodium borohydride, purum p.a., ≥96% (gas-volumetric)

H9151

Sigma-Aldrich

Hexadecyltrimethylammonium bromide, BioXtra, ≥99%

H6269

Sigma-Aldrich

Hexadecyltrimethylammonium bromide, for molecular biology, ≥99%

S8157

Sigma-Aldrich

Silver nitrate, BioXtra, >99% (titration)

A7506

Sigma-Aldrich

L-Ascorbic acid, reagent grade, crystalline

A0278

Sigma-Aldrich

L-Ascorbic acid, reagent grade

A2218

Sigma-Aldrich

L-Ascorbic acid, meets USP testing specifications

A4544

Sigma-Aldrich

L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%

A5960

Sigma-Aldrich

L-Ascorbic acid, BioXtra, ≥99.0%, crystalline

S7276

Sigma-Aldrich

Silver nitrate, BioReagent, suitable for plant cell culture, >99% (titration)

452882

Sigma-Aldrich

Sodium borohydride, powder, ≥98.0%

452890

Sigma-Aldrich

Sodium borohydride, caplets (18 × 10 × 8 mm), 98%

204390

Sigma-Aldrich

Silver nitrate, 99.9999% trace metals basis

452904

Sigma-Aldrich

Sodium borohydride solution, ~12 wt. % in 14 M NaOH

452874

Sigma-Aldrich

Sodium borohydride, granular, 10-40 mesh, 98%

52365

Sigma-Aldrich

Hexadecyltrimethylammonium bromide, BioUltra, for molecular biology, ≥99.0% (AT)

270997

Sigma-Aldrich

Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

215511

Sigma-Aldrich

Sodium borohydride solution, 2.0 M in triethylene glycol dimethyl ether

439223

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

213462

Sigma-Aldrich

Sodium borohydride, ReagentPlus^®, 99%

107700

Sigma-Aldrich

4-(Dimethylamino)pyridine, ReagentPlus^®, ≥99%

480886

Sigma-Aldrich

Sodium borohydride, granular, 99.99% trace metals basis

792276

Sigma-Aldrich

Silver nitrate, anhydrous, ≥99.999% trace metals basis

T8154

Sigma-Aldrich

Trypan Blue solution, 0.4%, liquid, sterile-filtered, suitable for cell culture

D1435

Sigma-Aldrich

Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications

D8418

Sigma-Aldrich

Dimethyl sulfoxide, for molecular biology

D4540

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture

D2650

Sigma-Aldrich

Dimethyl sulfoxide, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

D9170

Sigma-Aldrich

Dimethyl sulfoxide, PCR Reagent