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  • Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Journal of nanoscience and nanotechnology (2014-04-18)
Ying Sun, Liubin Qin, Dejun Liu, Changhong Liu, Yongwei Sun, Yourong Duan
PMID24738354
ABSTRACT

Colloidal gold is extensively used for molecular sensing because that the surface plasmon resonance (SPR) bands are affected by changes in the dielectric properties in the close vicinity of these structures due to the binding of ligands to the corresponding receptor molecules immobilized onto the nanostructures through chemi- or physisorption. We describe a simple method for the detection of Alpha-Fetoprotein-L3 which is a new generation of tumor marker for hepatocellular carcinoma (HCC) based on the aggregation of Lens culinaris agglutinin (LCA) Immobilized Gold Nanoparticles. The LCA conjugated GNPs obtained were 15-20 nm in diameter. The visible color change of the gold nanoparticles from purple to blue on interaction with 100 ng/mL of AFP-L3 is the principle applied here for the sensing of AFP-L3 level. UV/Vis spectroscopy also allows assay monitoring by quantifying the red shift of the plasmon resonance wavelength. With this method, the protein AFP-L3 can be rapidly detected as demanded for clinical diagnosis.

MATERIALS
Product Number
Brand
Product Description
GF43139538
Gold, insulated wire, 5m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
GF29872794
Gold, insulated wire, 1m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF81903753
Gold, tube, 50mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%
GF87786040
Gold, microfoil, disks, 10mm, thinness 0.75μm, specific density 1449μg/cm2, permanent mylar 3.5μm support, 99.99+%
GF21945113
Gold, insulated wire, 2m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
GF54558175
Gold, insulated wire, 0.2m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF11444611
Gold, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
GF55539524
Gold, tube, 100mm, outside diameter 1.0mm, inside diameter 0.7mm, wall thickness 0.15mm, as drawn, 99.95%
GF08843867
Gold, rod, 10mm, diameter 3.0mm, as drawn, 99.95%
GF12231223
Gold, microleaf disks, 10mm, thinness 0.25μm, specific density 483μg/cm2, removable support, 99.99+%
GF49538469
Gold, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%
GF58911206
Gold, tube, 200mm, outside diameter 0.3mm, inside diameter 0.2mm, wall thickness 0.05mm, as drawn, 99.95%
GF80583701
Gold, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.009mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF32175569
Gold, insulated wire, 1m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
GF41388767
Gold, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
GF06766713
Gold, insulated wire, 5m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%
GF70223408
Gold, tube, 50mm, outside diameter 1.0mm, inside diameter 0.7mm, wall thickness 0.15mm, as drawn, 99.95%
GF63042870
Gold, rod, 10mm, diameter 5.0mm, as drawn, 99.95%
GF66135641
Gold, microleaf disks, 10mm, thinness 1.0μm, specific density 1932μg/cm2, removable support, 99.99+%
GF18244661
Gold, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.009mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF82838379
Gold, insulated wire, 0.5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%
GF42441230
Gold, tube, 200mm, outside diameter 1.0mm, inside diameter 0.7mm, wall thickness 0.15mm, as drawn, 99.95%
GF18083178
Gold, tube, 50mm, outside diameter 1.60mm, inside diameter 0.6mm, wall thickness 0.5mm, as drawn, 99.95%
GF30409253
Gold, rod, 100mm, diameter 1.6mm, as drawn, 99.95%
GF62399133
Gold, insulated wire, 0.5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
GF68049827
Gold, tube, 200mm, outside diameter 1.60mm, inside diameter 0.6mm, wall thickness 0.5mm, as drawn, 99.95%
GF11345958
Gold, insulated wire, 1m, conductor diameter 0.05mm, insulation thickness 0.009mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF48164531
Gold, tube, 100mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%
GF98454864
Gold, rod, 25mm, diameter 1.6mm, as drawn, 99.95%
GF37867887
Gold, rod, 50mm, diameter 1.6mm, as drawn, 99.95%