gDNA Purification
DNA purification kits are used to extract and isolate DNA from samples, removing biological and chemical impurities such as cell debris, serum, proteins, lipids, and ionic components. DNA purification kits are also used to clean and prepare DNA samples for use in various downstream applications including cloning, gene editing, in situ hybridization, PCR, qPCR, multiplex PCR, sequencing, and next generation sequencing (NGS).
GenElute™ DNA Purification Kits
Classic GenElute™ DNA purification kits use a silica resin to strongly bind DNA under high salt conditions, allowing proteins, small RNAs, and other molecules to be removed through wash steps using a salt/ethanol solution. After washing, purified DNA is eluted in water or TE buffer. A broad portfolio of GenElute™ DNA purification kits tailored to specific sample types are available, enabling purification of genomic DNA (gDNA) from cell culture, mammalian tissue, blood, bacteria, viruses, plant tissue, soil, water, urine, and stool. GenElute™ Cell-Free DNA Kits provide rapid and efficient purification of circulating free DNA (cfDNA) to recover cell-free DNA fragments in the range of 100 bp – 500 bp, suitable for a wide range of downstream applications including next-generation sequencing, qPCR and bisulfite sequencing.
Features:
- Silica-based DNA purification method
- High yield, high purity DNA at lowest cost per prep
- Most common technique used for DNA purification in laboratory workflows
- ~90-minute protocol (varies based on lysis time)
- Comprehensive portfolio of kits tailored for specific sample types
- Vacuum and spin formats
- Multi-analyte (dual RNA/DNA copurification kits) available
GenElute™-E Single Spin DNA Purification Kits
GenElute™-E DNA purification kits use a negative chromatography technique dependent on size exclusion to isolate and purify nucleic acid in a single spin. In size exclusion chromatography, molecules are separated by size. Smaller molecules such as proteins, lipids, and ionic components become trapped in the pores of the stationary phase of the column. Larger DNA molecules flow through the column quickly because they are too large to enter the pores. By exploiting this difference in size, GenElute™-E purification kits enable DNA purification in a single spin without the need for multiple binding and wash steps.
Features:
- Negative chromatography DNA purification method based on size exclusion
- Simplified workflow
- Quick protocol (3 minutes hands-on time, for a total of 15-45 minutes depending on lysis time)
- Fewer chemical impurities, with no chaotropic salts or alcohol wash solutions that can inhibit downstream processes such as PCR
- Fewer biological impurities, with less shearing of genomic DNA due to reduced number of spin steps
- Reduced plastic waste and no hazardous binding or wash buffers
- Eco-friendly packaging
- Easy-to-follow protocols and checklists
- Portfolio of kits tailored for genomic DNA purification, PCR cleanup, and gel extraction
EXTRACT-N-AMP™ PCR KITS
Extract-N-Amp™ PCR kits are the first integrated DNA extraction and amplification kits for blood, tissue, and plant samples. These innovative kits provide all the reagents necessary to rapidly release DNA and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. Kits also include a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract.
Features:
- Integrated purification and amplification
- Simplified sample processing
- Kits include all reagents, proprietary buffers, and enzymes needed to rapidly extract and amplify targets of interest from a variety of cells and tissues
- REDExtract-N-Amp™ PCR ReadyMix™ contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reaction onto agarose gels for analysis
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