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SRP0336

Sigma-Aldrich

SYK Catalytic Subunit active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥60% (SDS-PAGE)

Synonym(s):

p72-Syk, spleen tyrosine kinase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥60% (SDS-PAGE)

form

aqueous solution

specific activity

≥139 pmol/min-μg

mol wt

58 kDa

packaging

pkg of 10 μg

technique(s)

activity assay: suitable
inhibition assay: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SYK(6850)

General description

Recombinant human SYK, (Genbank Accession No. NM_003177), amino acids 359-635 (end) with an N-terminal GST-tag, MW = 58 kDa, expressed in Sf9 insect cells.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Fomulated in 40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 4 mM glutathione, and 20% glycerol.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Tomoko Takano et al.
Antioxidants & redox signaling, 4(3), 533-541 (2002-09-07)
Oxidative stress induces the activation of multiple signaling pathways related to various cellular responses. In B cells, Syk has a crucial role in intracellular signal transduction induced by oxidative stress as well as antigen receptor engagement. Treatment of B cells
Christopher S Navara
Current pharmaceutical design, 10(15), 1739-1744 (2004-06-08)
The spleen tyrosine kinase Syk is an enigmatic protein tyrosine kinase functional in a number of diverse cellular processes. It is best known as a non receptor protein tyrosine kinase involved in signal transduction in cells of hematopoietic origin and

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