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MSRT2

Sigma-Aldrich

SigmaProt Intact Protein LC-MS Standard

Proteomics Retention Time Standard

Synonym(s):

Intact Protein LC-MS Standard, SigmaProtein Standard

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About This Item

EC Number:
UNSPSC Code:
23201100
NACRES:
NA.24

Quality Level

form

ready-to-use solution

storage temp.

−20°C

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Application

The RP-LC-MS Protein Standard is a LC-MS platform standard that is intended to test LC-MS system suitability and intact protein mass measurement. The proteins in MSRT2 are chosen to represent an elution profile for complex proteomic samples. The proteins have been selected based on UV and electrospray response, and are readily observed by both modes of detection. After reconstitution, MSRT2 is an injection-ready standard. MSRT2 can be utilized in studies such as in top-down proteomics.

Components

MSRT2 contains these 9 proteins:
  • Ribonuclease B
  • Insulin
  • Lysozyme
  • Transferrin
  • Bovine serum albumin (BSA)
  • Trypsin inhibitor
  • β-lactoglobulin A
  • Carbonic anhydrase
  • Lactate dehydrogenase

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Raffeal Bennett et al.
Journal of chromatography. A, 1523, 257-264 (2017-08-02)
Enhanced-fluidity liquid chromatography (EFLC) methods using methanol/H

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Step-by-step reversed phase UHPLC-MS workflow for middle-up mass analysis of an immunoglobulin G antibody, consisting of antibody purification, IdeS proteolysis and reduction, mass spectrometer calibration, mAb quantification, and a system suitability test.

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