GE28-9269-77

HiScreen^™ MabSelect^™ SuRe^™

Cytiva 28-9269-77

• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• ligand: alkali-stabilized protein A-derived (E. coli)
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 28-9269-77
• storage condition: (20% Ehtanol); (20% Ethanol)
• parameter: 44 psi
• bed size: 7.7 mm × 100 mm
• bed volume: 4.7 mL
• column I.D.: 7.7 mm
• matrix: highly cross-linked agarose
• avg. part. size: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-13.7
• working range: 3-12
• capacity: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• suitability: suitable for bioprocess medium
• storage temp.: 2-8°C

Description

General description

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiScreen^™ MabSelect^™ SuRe^™ is prepacked with MabSelect^™ SuRe^™ and is part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies (MAbs).

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

Lab Purification using the MabSelect^™ SuRe^™ Protein A tag for capture of antibodies.

Features and Benefits

• The novel, alkali-tolerant recombinant protein A ligand of MabSelect^™ Sure allows cleaning-in-place (CIP).
• Prepacked, ready-to-use columns for convenient process development.
• 10 cm bed height of HiScreen^™ columns is designed to allow method optimization and parameter screening.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.

HiScreen is a trademark of Cytiva

MabSelect is a trademark of Cytiva

SuRe is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids