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Key Documents

CWDS-MF780

Sigma-Aldrich

ColorWheel® Antibody-Ready mFluor Red 780 Dye

greener alternative

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

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About This Item

UNSPSC Code:
41116015

product line

ColorWheel®

Quality Level

form

lyophilized

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

flow cytometry: suitable

fluorescence

λex 629 nm; λem 767 nm

compatibility

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

greener alternative category

shipped in

ambient

storage temp.

2-8°C

General description

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.

mFluor Red 780 is a water-soluble dye that exhibits an excitation at 629 nm and emission at 767 nm with an extinction coefficient of 90,000 cm-1 M-1. Its spectral properties are comparable to APC-Alexa Fluor 750. However, compared to APC-Alexa Fluor 750, it is more photostable and its conjugates are suitable for use in Flow Cytometry.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Application

Quality Control Testing:

Flow Cytometry: Excitation: 629 nm, Emission: 767 nm

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel® Antibody (Sold Separately)

Physical form

CWDS-MF780-25UL Set Components

CWD-MF780-25UL - ColorWheel® Antibody-Ready mFluor Red 780 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL - ColorWheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitution

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Find answers to frequently asked questions (FAQs) about ColorWheel® flow cytometry antibodies and dyes, straight from the experts.

Harness the flexibility of ColorWheel® flow cytometry antibodies and dyes to unlock freedom in your flow cytometry multiplex assay design or multicolor panel design and simplify your multiplex flow cytometry workflows.

Protocols

Experience simplicity in your flow cytometry workflow with the 3-Step ColorWheel® Flow Cytometry Reagent Preparation protocol. With less than 5 minutes of hands-on time, see how simple it is to create your own optimal flow cytometry reagents with ColorWheel® flow cytometry antibodies and dyes.

View ColorWheel® protocol steps for flow cytometry analysis when using ColorWheel® antibodies with ColorWheel® dyes including antibody preparation, PBMC sample preparation, cell surface staining, and intracellular (cytoplasmic) staining.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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