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P5936

Sigma-Aldrich

Pectolyase from Aspergillus japonicus

greener alternative

suitable for plant cell culture, lyophilized powder

Synonym(s):

PEL1

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About This Item

Enzyme Commission number:
3.2.1.15 (BRENDA, IUBMB)
MDL number:
MFCD00131813
UNSPSC Code:
10171502
NACRES:
NA.72

form

lyophilized powder

Quality Level

200

specific activity

≥0.3 unit/mg solid

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

cell culture | plant: suitable

application(s)

agriculture

greener alternative category

Enabling,

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Pectolyase P5936 (Pel1) is a natural mixed pectolyase produced by the fungus Aspergillus japonicus used to digest components (endopolygalacturonate and pectin) of plant cell walls. Treatment of cell walls with pectolyase can be used to destablilize the cell walls for organelle isolation or to modify the elasticity of the cell walls.
Pectolyase from Aspergillus japonicas has been used to digest the siliques and also in immunofluorescence experiments.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Other Notes

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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AtKP1, a kinesin-like protein, mainly localizes to mitochondria in Arabidopsis thaliana
Ni C Z, et al.
Cell Research, 15(9), 725-725 (2005)
Nuclear fusions contribute to polyploidization of the gigantic nuclei in the chalazal endosperm of Arabidopsis
Baroux C, et al.
Planta, 220(1), 38-46 (2004)
Qingxin Zhao et al.
Plant physiology, 147(4), 1874-1885 (2008-06-20)
Mature plant cell walls lose their ability to expand and become unresponsive to expansin. This phenomenon is believed to be due to cross-linking of hemicellulose, pectin, or phenolic groups in the wall. By screening various hydrolytic enzymes, we found that
Detection of the quantity of kinesin and microgravity-sensitive kinesin genes in rat bone marrow stromal cells grown in a simulated microgravity environment
Ni C, et al.
Acta Astronautica, 68(11-12), 1722-1728 (2011)
Sara Posé et al.
Journal of experimental botany, 64(12), 3803-3815 (2013-07-23)
Antisense-mediated down-regulation of the fruit-specific polygalacturonase (PG) gene FaPG1 in strawberries (Fragaria×ananassa Duch.) has been previously demonstrated to reduce fruit softening and to extend post-harvest shelf life, despite the low PG activity detected in this fruit. The improved fruit traits
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