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F7264

Sigma-Aldrich

Fixing solution

5 × (solution)

Synonym(s):

pre-stain fixing solution

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

concentration

5 × (solution)

technique(s)

protein staining: suitable

Application

An effective protein fixing solution for pre-stain fixing, suitable for SDS-PAGE, non-denaturing PAGE and IEF. Proteins require fixing into the gel for analytical purposes so that they do not leach out following electrophoresis.

Other Notes

60% (w/v) trichloroacetic acid, 17.5% (w/v) 5-sulfosalicylic acid.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Articles

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.

Protocols

Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximum sensitivity.

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