MAB5258
Anti-Synaptophysin Antibody, clone SY38
clone SY38, Chemicon®, from mouse
Synonym(s):
synaptophysin, Synaptophysin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
SY38, monoclonal
species reactivity
bovine, rat, avian, fish, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SYP(6855)
Related Categories
General description
Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non chromaffin paragangliomas.
Specificity
The antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12.
Immunogen
Vesicular fraction of bovine brain. The SY38 epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus & Betz 1990).
Application
Detect Synaptophysin using this Anti-Synaptophysin Antibody, clone SY38 validated for use in FC, IC, IH & WB.
Flow Cytometry (FACS):
A previous lot of this antibody was used in Flow Cytometry.
Western Blot:
(Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein
Immunohistochemistry:
For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used.
It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution.
Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in Flow Cytometry.
Western Blot:
(Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein
Immunohistochemistry:
For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used.
It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution.
Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
Quality
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates.
Target description
38 kDa
Linkage
Replaces: 04-1019
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1κ in buffer containing 20 mM sodium phosphate buffer, 0.25 M NaCl, containing 0.1% sodium azide.
Storage and Stability
Stable for 6 months at 2-8ºC from date of receipt. DO NOT FREEZE.
Analysis Note
Control
Positive Control: Pancreas tissue
Negative Control: Normal mouse serum
Rat hippocampus tissue, mouse brain lysate.
Positive Control: Pancreas tissue
Negative Control: Normal mouse serum
Rat hippocampus tissue, mouse brain lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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