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HPLC Analysis of Proline Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive)

HPLC Analysis of Proline Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Astec® CHIROBIOTIC® T Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

guard cartridge

Product No.
Description
Pricing

Astec® CHIROBIOTIC® T Chiral HPLC Guard Column

Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

CONDITIONS

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (40:60:0.02, A:B:C)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 205 nm

injection

10 μL

sample

200 μg /mL in 50:50 water:methanol

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC