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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
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Triphenyl phosphate solution, NMR reference standard, 0.0485 M in acetone-d6 (99.9 atom % D), NMR tube size 10 mm × 8 in.
Sigma-Aldrich
Triphenyl phosphate solution, NMR reference standard, 0.0485 M in acetone-d6 (99.9 atom % D), NMR tube size 3 mm × 8 in.
Supelco
Triphenyl phosphate solution, NMR reference standard, 3 mM in chloroform-d (99.8 atom % D), NMR tube size 5 mm × 8 in.
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Residual Solvent - Chloroform, Pharmaceutical Secondary Standard; Certified Reference Material
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Ammonium hydroxide solution, BioUltra, ~1 M NH3 in H2O (T)
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4-Methylmorpholine, BioXtra, suitable for protein sequencing, ≥99.5% (GC)
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Calcium chloride, AnhydroBeads, −10 mesh, ≥99.99% trace metals basis
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Pyridine, ≥99%
Supelco
Toluene, analytical standard
Supelco
Triphenyl phosphate, analytical standard
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Sigma-Aldrich
Triphenyl phosphate solution, NMR reference standard, 0.0485 M in acetone-d6 (99.9 atom % D)
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Ammonium hydroxide solution, ACS reagent, 28.0-30.0% NH3 basis
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Ammonium hydroxide solution, 28% NH3 in H2O, ≥99.99% trace metals basis
Sigma-Aldrich
Toluene, ACS reagent, ≥99.5%
Sigma-Aldrich
Pyridine, biotech. grade, ≥99.9%
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Toluene, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Methyl alcohol, United States Pharmacopeia (USP) Reference Standard
Benzyl alcohol, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Pyridine, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Toluene, ACS reagent, ≥99.5%
Sigma-Aldrich
Toluene, ACS reagent, ≥99.5%
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Calcium chloride
Supelco
Calcium ion solution for ISE, 0.1 M Ca, for ion-selective electrodes, analytical standard (for ion-selective electrodes)
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Benzyl alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
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N,N-Diisopropylethylamine, ReagentPlus®, ≥99%
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N,N-Diisopropylethylamine, 99.5%, biotech. grade
Sigma-Aldrich
N,N-Diisopropylethylamine, purified by redistillation, 99.5%