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Single Molecule Counting (SMC®) Technology

Originally developed by Singulex®, Inc., Single Molecule Counting (SMC®) technology with the SMCxPRO® platform enables researchers to measure femtogram levels of biomarkers and better understand the clinical relevance from healthy to disease. Learn more about SMC® technology, immunoassay kits, and how you can design your own custom assay to meet your critical research needs. 

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Digital Counting Improves Sensitivity and Dynamic Range

Providing maximum immunoassay performance while following a workflow similar to traditional ELISA technology, SMC® technology combines a unique assay elution step and robust digital counting to achieve improved signal-to-noise ratios over traditional immunoassay technologies. The SMC® technology thus provides enhanced quantification at both low and high levels of expression on one complete system. The SMCxPRO® instrument captures the sum of all digital events counted above the background threshold. The digital event algorithm of the SMCxPRO® instrument computes digital events across a spectrum of time series for a single standard. The digital counting of molecules thus improves the assay sensitivity and extends the assay dynamic range beyond what can be achieved with traditional methods.

Verification Criteria for SMC® Immunoassays

A comprehensive set of criteria evaluating ultimate performance are used to qualify verified SMC® immunoassays, including:

  • Lower limit of quantification
  • Inter- and intra-assay precision
  • Spike recovery
  • Dilutional linearity and parallelism

Related Product Resources

Learn more about how SMC® technology can take your research to the next level by exploring the topics below.

SMC® Research Areas

Browse all the ways researchers are using SMC® technology including drug development, neuroscience, cardiotoxicity, immunology research, and more.

Browse Research Areas >

Why Choose SMC® Technology?

See why choosing ultrasensitive SMC® technology can help you take a deeper look at your research to discover even more.

See Why >

SMCxPRO® Platform

SMC® assays are run on the state-of-the-art SMCxPRO® detection instrument. Discover more about this ultrasensitive immunoassay platform.

Discover More >

Other SMC® Instrumentation

View info on ancillary kits and equipment for SMC® assays that help optimize your workflows.

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Custom Assay Services

Explore more about our Custom Assay Development & Innovation (CADI) services and request more info.

Explore Services >

Tips and Support for SMC® Products

Get tips and tricks for running SMC® assays straight from the experts.

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Subscribe to Our Newsletter

Stay up to date on the latest immunoassay research trends and our newest product offerings by subscribing to our Analyte Update newsletter or explore our current immunoassay offerings in the Analyte Quarterly.

Related Videos

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How to Seal Read Plates for the SMCxPRO<sup>®</sup> Platform
How to Seal Read Plates for the SMCxPRO® Platform
Learn how to properly seal the 384-well read plates for the SMCxPRO® platform in four easy steps.
How to Pipette for Successful Assays
How to Pipette for Successful Assays
Get expert tips for forward and reverse pipetting for the most successful SMC® ultrasensitive assays.
SMCxPRO<sup>®</sup> Instrument Set-up and Calibration
SMCxPRO® Instrument Set-up and Calibration
Learn how to initialize and calibrate your SMCxPRO® instrument.
How to Reconstitute the IL-6 Standard
How to Reconstitute the IL-6 Standard
Learn how to reconstitute the IL-6 standard for the SMC®platform.
How to Prepare the IL-6 Standard Curve
How to Prepare the IL-6 Standard Curve
Discover how to prepare the IL-6 standard curve using SMC® technology.
How to Prepare and Dispense the IL-6 Beads
How to Prepare and Dispense the IL-6 Beads
Watch how to prepare and dispense the IL-6 beads for SMC® assays.
How to Prepare and Dispense the Detection Antibody
How to Prepare and Dispense the Detection Antibody
Watch how to prepare and dispense the detection antibody for SMC® assays.
Elution and Neutralization Steps
Elution and Neutralization Steps
View key elution and neutralization steps in the SMC® protocol.
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