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G1006564

AOAC Method 2012.13: GC Analysis of Fatty Acids in Powdered Infant Formula on SP®-2560

suitable for GC

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technique(s)

gas chromatography (GC): suitable

test parameters

sample/matrix: weigh 168 mg powdered infant formula (approximately 50 mg of fat) into a 25 mL centrifuge tube; add 2 mL of water and mix to dissolve; (let sit for 15 minutes at room temperature; add 5 mL of internal standard (C11:0 FAME + C13:0 TAG, each at 2 mg/mL in methyl tert butyl ether))
extraction: 5 mL of 5% (w/v) methanolic sodium methoxide solution; close tube and vortex for 10 seconds; after 180 seconds (time starts when sodium methoxide is added) add 2 mL of hexane; (after 210 seconds add 10 ml of neutralization solution (10% disodium hydrogen citrate/15% sodium chloride in water); gently shake using vortex mixer; centrifuge mixture at 1750 rpm for 5 minutes)
eluate post-treatment: transfer 200 μL of supernatant into 10 mL flask and dilute to mark with hexane
column: SP-2560, 100 m x 0.25 mm I.D., 0.20 μm (24056)
oven: 60 °C (1 min), 15 °C/min to 165 °C (1 min), 2 °C/min to 225 °C (20 min)
inj. temp.: 250 °C
detector: FID, 250 °C
carrier gas: helium, 0.8 mL/min
injection: 1μL, 10:1 split
liner: 4 mm I.D., split/splitless type, wool packed single taper FocusLiner™ design

application(s)

food and beverages

Analysis Note

The nutritional content of infant formula is extremely critical, and thus is one of the most regulated food products worldwide. Most commercial infant formulas are derived from cow′s milk, and are formulated to mimic human breast milk. They offer the advantage of being nutritionally balanced and easy for most babies to digest. All infant formulas, whether cow′s milk-based or from another source, are subject to specific regulatory nutritional content requirements. AOAC Method 2012.13 using a 100 m SP-2560 is able to provide identification of fatty acids, as well as determination of total fatty acid content and breakdown by class.

Legal Information

FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SP is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    C8:0
    C10:0
    C11:0 (I.S.)
    C12:0
    C13:0 (I.S.)
    C14:0
    C14:1n5
    C15:0
    C16:0
    C16:1n7
    C17:0
    C17:1n7
    C18:0
    C18:1 trans
    C18:1 cis
    C18:2n6
    C18:2n7
    C18:3n6
    C18:3n3
    C20:0
    C20:1n9
    C20:2n6
    C20:3n3
    C20:4n6
    C22:0
    C22:1n9
    C22:6n3 (DHA)
    C24:0
    See All (28)

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