T8076

Anti-TRB3 (N-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Human tribbles 3 homolog, Anti-NIPK, Anti-SINK, Anti-SKIP3, Anti-TRIB3
• MDL number: MFCD08705525
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 39 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): western blot: 1-2 μg/mL using HEK-293 cells expressing human TRB3
• UniProt accession no.: Q96RU7
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TRIB3(57761)

Description

General description

Tribbles homolog 3 (TRB3) gene is located on the 20p13 human chromosome. TRB3 and its related family members TRB1 and TRB2 show 45% sequence identity. It exhibits resemblance to ′tribbles′, a drosophila protein. TRB family members have a truncated kinase domain that lacks an adenosine 5′-triphosphate binding site (GXGXXG) and contain a variant catalytic core motif.

Immunogen

synthetic peptide corresponding to amino acids 11-29 located near the N-terminus region of human TRB3. The immunizing sequence has limited homology (~50% identity) to mouse and rat TRB3, and has no identity to TRB1 and TRB2 isoforms.

Application

Anti-TRB3 (N-terminal) antibody produced in rabbit has been used in immunoprecipitation and immunoblotting.

Biochem/physiol Actions

Tribbles homolog 3 (TRB3) like tribbles has also been identified as a potential pro-apoptotic protein, and is induced by a variety of cell stress conditions, including hypoxia and nutrient starvation. It was previously identified as a neuronal cell death-inducible protein kinase (NIPK) that is rapidly upregulated during neuronal apoptosis. TRB3 binds to activating transcription factor 4 (ATF4) and inhibits its transcriptional activity. TRB3 has been shown to interact with mitogen-activated protein kinases (MAPK) kinases to regulate activation of MAPK pathways.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable