NP cl.25
Reactivity: Anti-Measles Virus Nucleoprotein, 95051114
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About This Item
UNSPSC Code:
41106514
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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0 Ag-14
Reactivity
Anti-Measles Virus Nucleoprotein
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Sp2/0 x Mouse BALB/c spleen
Cell Line Description
NP cl.25 monoclonal antibody was produced in vitro by fusing mouse myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. NP cl.25 is negative for haemagglutination and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue. It has been used for measuring synthesis and topological accumulation of measles virus antigen during the lytic cycle.
Immunogen
Measles virus (strain Hallè)
Application
Diagnosis, study of measles virus infection
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37°C
Other Notes
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Certificates of Analysis (COA)
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