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Key Documents

Z374873

PCR microtubes with attached caps

capacity 0.2 mL

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About This Item

UNSPSC Code:
41121702
NACRES:
NB.22

material

colorless cap
colorless tube
conical bottom
flat cap
polypropylene
polypropylene cap
polypropylene tube

description

Autoclavable

sterility

non-sterile

feature

RNase and DNase free
autoclavable at 121 °C
graduations: no
Push fit

packaging

pack of 250 ea
case of 4 packs

technique(s)

PCR: suitable

capacity

0.2 mL

color

clear

suitability

suitable for (PCR applications)

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General description

Thin-walled for efficient thermal transfer and shorter cycle times; fits all leading thermal cyclers including PerkinElmer, Biometra, MJ Research, Techne, Grant and Strategene (0.65 mL only). Certified DNase- and RNase-free. All tolerate organic solvent reactions and temperatures from -4 to 121 °C.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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