N1531
Denaturation Solution
for Northern and Southern blotting
Synonym(s):
Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.25
General description
Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.
Application
Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.
Components
The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.
Other Notes
0.5 N NaOH, 1.5 M NaCl
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
Storage Class Code
8B - Non-combustible, corrosive hazardous materials
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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