1.50646

Purospher^® STAR RP-18 endcapped (2 µm)

L × I.D. 50 mm x 2.1 mm Hibar^® HR UHPLC column

• Synonym(s): Purospher^®STAR RP-18 endcapped (2µm) Hibar^® HR 50-2.1
• UNSPSC Code: 41115709

Properties

• material: stainless steel column
• Quality Level: 100
• Agency: suitable for ASTM^® 7968; suitable for ASTM^® 7979; suitable for EPA 1633; suitable for EPA 38407-42; suitable for EPA 533; suitable for EPA 537.1; suitable for EPA 8327; suitable for EPA ACB B21-02; suitable for EPA ACB B23-05b; suitable for GB 5009.253-2016; suitable for ISO 21675 2019; suitable for ISO 25101; suitable for ISO/CEN 15968-2010; suitable for USP L1
• product line: Purospher^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 17% Carbon loading
• parameter: 1000 bar pressure; 65 °C max. temp.
• technique(s): LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 5 cm × 2.1 mm
• surface area: 330 m²/g
• matrix: fully porous particle
• matrix active group: C18 bonding phase
• particle size: 2 μm
• pore size: 120 Å pore size
• operating pH range: 1.5-10.5
• separation technique: reversed phase; supercritical fluid (SFC)
• storage temp.: no temp limit

Description

General description

Purospher^® STAR RP-18 endcapped, 2 µm UHPLC columns are designed for use in UHPLC Applications and instruments providing full UHPLC resolution and highest separation efficiency′s at maximum pressure of 1000 bar.Purospher^® STAR RP-18 endcapped, 2 µm UHPLC columns allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher^® STAR RP-18 endcapped, 2 µm Hibar^® HR 50-2.1 is a ready to use UHPLC column suitable for connection to any UHPLC system providing a pressure stability of 1000 bar.The column dimension of 50 mm length x 2.1 mm I.D. is the optimal choice for high throughput UHPLC separation of complex samples allowing significant solvent savings and improved sensitivity.Learn more about Purospher^® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

• Ultra-pure silica (99.999%) for excellent peak symmetry
• High separation efficiency
• Reproducible results from run-to-run and batch-to-batch
• Best all-round performance according to Tanaka
• Outstanding pH stability from pH 1.5 to 10.5
• No phase collapse when using highly aqueous mobile phases
• Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 140 bar
Theoretical Plates (N/m) (1,2-Benzanthracene): ≥ 170000
Symmetry (1,2-Benzanthracene): 0.8 - 1.4
Capacity factor (1,2-Benzanthracene): 8.5 - 11.5

Legal Information

ASTM is a registered trademark of American Society for Testing and Materials

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany