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1.15933

Sigma-Aldrich

Carmine (C.I. 75470)

(calcium-aluminium lacquer of carminic acid) for microscopy Certistain®

Synonym(s):

Carmine (C.I. 75470)

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About This Item

Empirical Formula (Hill Notation):
C44H37AlCaO27 · 3H2O
CAS Number:
Molecular Weight:
1118.85
UNSPSC Code:
41116130
EC Index Number:
215-724-4

Quality Level

form

solid

composition

Dye, ≥50% spectrophotometric assay

solubility

practically insoluble 1.3 g/L

bulk density

290 kg/m3

εmax

105-150 at 525-532 nm in DMSO at 0.05 g/L
80-110 at 565-570 nm in DMSO at 0.05 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C22H20O13/c1-4-8-5(2-6(24)9(4)22(33)34)13(25)10-11(15(8)27)16(28)12(18(30)17(10)29)21-20(32)19(31)14(26)7(3-23)35-21/h2,7,14,19-21,23-24,26,28-32H,3H2,1H3,(H,33,34)

InChI key

DGQLVPJVXFOQEV-UHFFFAOYSA-N

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General description

Carmine (C.I. 75470) (calcium-aluminium lacquer of carminic acid) for microscopy Certistain®, is a dry dye that is used for the preparation of a staining solution for staining nuclei and for demonstration of glycogen in histological sections of human origin. As a Certistain® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Identity
- aluminium:
passes test
- Calcium: passes test
- UV/VIS-Spectrum: passes test
Dye content (spectrophotometrically): ≥ 50 % (als C₂₂H₂₀O₁₃)
Absorption maximum λ 1 (Dimethyl sulfoxide): 565 - 570 nm
Absorption maximum λ 2 (Dimethyl sulfoxide): 525 - 532 nm
Spec. Absorptivity A 1%/1cm
- λ1max; 0.05 g/l; DMSO:
80 - 110
- λ2max; 0.05 g/l; DMSO: 105 - 150
TLC-Test: passes test
Water (according to Karl Fischer): ≤ 15 %
Sulfated ash: 10 - 17 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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