04-745
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal
culture supernatant, clone MC315, Upstate®
Synonym(s):
H3K4me3, Histone H3 (tri methyl K4)
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
MC315, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
multiplexing: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
trimethylation (Lys4)
Gene Information
human ... H3C1(8350)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Specificity
Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See COA for specificity data.
Immunogen
Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
Application
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315 is a rabbit monoclonal antibody for detection of trimethyl-Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in WB, ChIP, DB, Mplex, ChIP-seq.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in human colon and human breast cancer tissue sections
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in HeLa, A431, HUVEC, and NIH3T3 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in HeLa, A431, HUVEC, and NIH3T3 cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Quality
routinely evaluated by immunoblot on in acid extracted proteins from HeLa cells, but not recombinant unmethylated Histone H3 (Catalog #14-494)
Target description
17 kDa
Linkage
Replaces: 04-791
Physical form
Cultured supernantant in 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa acid extract
HeLa acid extract
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Effects of chronic exposure to arsenic and estrogen on epigenetic regulatory genes expression and epigenetic code in human prostate epithelial cells.
Treas, JN; Tyagi, T; Singh, KP
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Antagonistic roles for BRM and BRG1 SWI/SNF complexes in differentiation.
Flowers, S; Nagl, NG; Beck, GR; Moran, E
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Chromatin interaction analysis using paired-end tag sequencing.
Melissa J Fullwood,Yuyuan Han,Chia-Lin Wei,Xiaoan Ruan,Yijun Ruan
Current Protocols in Molecular Biology null
Nucleosome positioning and histone modifications define relationships between regulatory elements and nearby gene expression in breast epithelial cells.
Rhie, SK; Hazelett, DJ; Coetzee, SG; Yan, C; Noushmehr, H; Coetzee, GA
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Senescence and dysfunction of proximal tubular cells are associated with activated p53 expression by indoxyl sulfate.
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