ALD00344
PTAD-Azide
95%
Synonym(s):
4-(4-(2-Azidoethoxy)phenyl)-1,2,4-triazolidine-3,5-dione, N3-Ph-Ur for e-Y-CLICK
About This Item
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Quality Level
Assay
95%
form
powder or crystals
reaction suitability
reagent type: cross-linking reagent
functional group
azide
storage temp.
2-8°C
SMILES string
O=C(NNC1=O)N1C2=CC=C(OCCN=[N+]=[N-])C=C2
InChI
1S/C10H10N6O3/c11-15-12-5-6-19-8-3-1-7(2-4-8)16-9(17)13-14-10(16)18/h1-4H,5-6H2,(H,13,17)(H,14,18)
InChI key
MHGMHPVYCVQIET-UHFFFAOYSA-N
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General description
Application
Note: PTAD-Azide must be first activated by stirring in a 1:0.98 molar ratio with 1,3-dibromo-5,5-dimethylhydantoin (product # 157902). Activation is evident upon solution color change from colorless to deep red and the activated reagent should be used immediately.
General Procedure for Protein Modification with PTAD.
Part 1: PTAD activation
- Mix together 1:0.98 molar equivalents of unactivated PTAD to 1,3-dibromo-5,5-dimethylhydantoin (product # 157902) in organic solvent (preferred solvents are DMF or acetonitrile, avoid using DMSO)
- Color change is observed from colorless/pale yellow to deep red (approximately 5 min of mixing).
- After the solution turns red, store the now activated reagent on ice and use for protein modification within 30 min.
Part 2: Protein modification
- Add protein solution in mixed phosphate/Tris buffer or Tris buffer (pH should be 6 - 9) to the eppendorf tube (or other vial) containing the activated PTAD reagent prepared above and mix gently at room temperature for up to 30 min. Preferably use 10-fold molar excess of reagent relative to protein. Use protein at a minimum concentration of 1 mg/ml (higher concentrations are preferred for enhanced labeling).
- Remove excess unreacted PTAD by gel filtration.
related product
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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