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581616-U

Supelco

Ascentis® Phenyl HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

Materiales

stainless steel column

Nivel de calidad

Agency

suitable for USP L11

Línea del producto

Ascentis®

Características

endcapped

fabricante / nombre comercial

Ascentis®

envase

1 ea of

Extensión del etiquetado

19% Carbon loading

Parámetros

≤70 °C temp. range
400 bar pressure (5801 psi)

técnicas

HPLC: suitable
LC/MS: suitable

L × D.I.

15 cm × 4.6 mm

superficie

450 m2/g

cobertura de la superficie

5.2 μmol/m2

impurezas

<5 ppm metals

matriz

fully porous particle
silica gel high purity, spherical

grupo activo de la matriz

phenyl phase

tamaño de partícula

5 μm

tamaño de poro

100 Å

operating pH range

2-8

aplicaciones

food and beverages

técnica de separación

reversed phase

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Descripción general

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis Phenyl provides superior separations in reversed-phase mode including 100% aqueous conditions. It may also be used in HILIC/ANP (aqueous normal phase) mode and shows low UV/MS bleed for gradient applications.

Características y beneficios

  • 100% Aqueous Compatible
  • ANP/HILIC and reversed-phase
  • Low UV/MS bleed for gradient applications
  • Alternate selectivity

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Información legal

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Visite la Librería de documentos

C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar

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