SAB4200107

Anti-CHD4 antibody, Mouse monoclonal

clone 3F2/4, purified from hybridoma cell culture

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: 3F2/4, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~250 kDa
• reactividad de especies: human, mouse
• concentración: ~1.0 mg/mL
• técnicas: immunoprecipitation (IP): suitable; western blot: 0.5-1.0 μg/mL using HeLa cell extracts
• isotipo: IgG2a
• Nº de acceso UniProt: Q14839
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CHD4(1108)

Descripción

Descripción general

Monoclonal Anti-CHD4 (mouse IgG2a isotype) is derived from the hybridoma 3F2/4 produced by the fusion of mouse myeloma cells and splenocytes from CD-1 mice immunized with a synthetic peptide derived from human chromodomain helicase DNA binding protein 4 (CHD4) conjugated to keyhole limpet hemocyanine (KLH). CHD4 is one of the subunits of Mi-2, an ATP-dependent helicase. It belongs to the sucrose non-fermentable (SNF2)/Rad54 helicase family of proteins. The CHD4 gene is mapped to human chromosome 12p13.31 and is highly conserved among animals and plants.

Inmunógeno

synthetic peptide derived from human CHD4, conjugated to KLH

Aplicación

Monoclonal Anti-CHD4 antibody produced in mouse has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation

Acciones bioquímicas o fisiológicas

Chromodomain helicase DNA binding protein 4 (CHD4) interacts with metastasis-associated protein (MTA1/2), histone deacetylase 1 (HDAC1) and RbAp46 in the nucleosome remodelling and deacetylation (NuRD) complex. Interestingly, antibodies against CHD4 have been found to be involved in the onset of dermatomyositis (DM), an autoimmune disease which is a form of idiopathic inflammatory myopathy (IIM). Variation in the gene due to mutation is observed in endometrial cancer. CHD4 regulates homologous recombination and thus maintains genome stability.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable