S7570

SGK2 Active human

≥75% (SDS-PAGE), aqueous glycerol solution, recombinant, expressed in E. coli

• Sinónimos: Serum/Glucocorticoid-Regulated Kinase 2
• Número MDL: MFCD07371188
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• recombinante: expressed in E. coli
• Nivel de calidad: 200
• Análisis: ≥75% (SDS-PAGE)
• formulario: aqueous glycerol solution
• actividad específica: 100 units/mg protein (Bradford)
• mol peso: protein apparent mol wt ~66 kDa
• Nº de acceso UniProt: Q9HBY8
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... SGK2(10110)

Descripción

Aplicación

Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Acciones bioquímicas o fisiológicas

SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C and has SGK2 enhances Na⁺/K⁺-ATPase activity in a variety of cells. In addition, SGK2 mimics the function of SGK1 and SGK3 and participates in the regulation of renal epithelial Na⁺ channel ENaC activity.

Definición de unidad

One unit will incorporate one nanomole of phosphate into the AKT/SGK substrate peptide(RPRAATF) per minute at 30 °C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

Forma física

Supplied at 50 μL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable