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GE28-9365-44

HiPrep™ Sp Fast Flow 16/10

Cytiva 28-9365-44

Sinónimos:

Fast Flow Column, Fast Flow SP Column, HiPrep SP Column

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About This Item

Código UNSPSC:
47101511
NACRES:
NA.56

ligand

sulphopropyl

descripción

Ion Exchanger Type (value)

envase

pkg of 1 ea

fabricante / nombre comercial

Cytiva 28-9365-44

Parámetros

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

lecho tamaño

16 mm × 100 mm

lecho volumen

20 mL

columna D.I.

16 mm

matriz

6% cross-linked agarose

tamaño de partícula

45-165 μm

diámetro promedio

90 μm

cleaning

3-14

working range

4-13

idoneidad

suitable for bioprocess medium

Descripción general

HiPrep™ SP FF is a strong cation exchanger prepacked with SP Sepharose™ Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.

HiPrep™ SP FF are prepacked, ready to use columns for ion exchange chromatography. They provide fast, preparative separations of proteins and other biomolecules.

Características y beneficios

  • HiPrep™ columns provide fast, reliable, and reproducible preparative ion exchange separations.
  • Good flow rates and high capacity.
  • The industry standard for ion exchange chromatography.
  • Compatible with single-pump configurations and chromatography systems such as AKTA design.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Otras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Información legal

HiPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins with products from GE Healthcare.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ion Exchange Chromatography Troubleshooting
Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ver todo

Protocolos

Refolding using ion exchange chromatography and analysis of refolding

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

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