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GE17-1279-02

rProtein A Sepharose™ Fast Flow

Cytiva 17-1279-02, pack of 25 mL

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

envase

pack of 25 mL

fabricante / nombre comercial

Cytiva 17-1279-02

condiciones de almacenamiento

(20% Ehtanol)

matriz

4% cross-linked agarose

tamaño de partícula

60-165 μm

diámetro promedio

90 μm (d50v)

cleaning in place

2-11

working range

3-10

capacidad

~50 mg binding capacity (human lgG/ml medium)

idoneidad

suitable for bioprocess medium

temp. de almacenamiento

2-8°C

Descripción general

rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale.

Monoclonal and polyclonal antibody purification

rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.

Protein A resin characteristics

The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.

Streamlined scale up

Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.

Características y beneficios

  • For monoclonal and polyclonal antibody purification at both laboratory and process scale.
  • Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes.
  • Free from animal-derived components.
  • Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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