E2156
Anti-EGF Receptor antibody, Mouse monoclonal
clone 225, purified from hybridoma cell culture
Sinónimos:
Anti-EGFR, Anti-Epidermal Growth Factor Receptor
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About This Item
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
225, monoclonal
formulario
buffered aqueous solution
reactividad de especies
human
concentración
~1.5 mg/mL
técnicas
immunoprecipitation (IP): 4-8 μg using cell lysate of A431 cells
isotipo
IgG1
Nº de acceso UniProt
aplicaciones
research pathology
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... EGFR(1956)
Categorías relacionadas
Descripción general
Monoclonal Anti-EGFR (mouse IgG1 isotype) is derived from the hybridoma 225 produced by the fusion of mouse myeloma cells (NS-1-503 cells) and splenocytes from BALB/c mice immunized with partially purified EGF receptors from A-431 cells. The receptor for epidermal growth factor (EGF) is an integral cell membrane glycoprotein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. The EGF receptor has an intracellular domain that exhibits tyrosine kinase activity.
Inmunógeno
partially purified EGF receptors from human A-431 cells.
Aplicación
Monoclonal Anti-EGF Receptor antibody has been used in antibody-nanoparticle conjugation.
Monoclonal Anti-EGF Receptor antibody is suitable for use in immunoprecipitation (4-8 μg using cell lysate of A431 cells).
Acciones bioquímicas o fisiológicas
EGFR (epidermal growth factor receptors) protein tyrosine kinase is activated when EGF binds the extracellular binding domain. The first detectable response is the autophosphorylation of the C-terminal tyrosine followed by phosphorylation of other endogenous substrates.
EGFR is a tyrosine kinase receptor that regulates cellular several functions such as growth, blood vessel formation, metastasis and invasion. Alterations in EGFR expression have been associated with a wide range of cancers. Thus, drugs that target EGFR signaling have important therapeutic applications in cancer .
Forma física
0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.
Información legal
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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