HT 1197

87032403, human bladder, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: HT 1197, 87032403
• origen biológico: human bladder
• modo de crecimiento: Adherent
• cariotipo: 2n = 46
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian bladder carcinoma

Descripción línea celular

Derived from a grade 4 transitional cell carcinoma of the bladder of a 44 year old Caucasian male who had not received chemo or radiation therapy. The cells grow in soft agar, produce fibrinolytic activity and are tumorigenic in mice and hamsters.

Aplicación

HT 1197 has been used to study the transcription activity of human endogenous retroviruses (HERV).

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 16,18

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2x10,000 to 5x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.