C-12211

Human Dermal Blood Endothelial Cells (HDBEC)

Juvenile foreskin, 500,000 cryopreserved cells

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human foreskin (juvenile dermis)
• envase: pkg of 500,000 cells
• morfología: ( endothelial)
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción general

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Origen línea celular

Skin

Aplicación

Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin from a single donor and are provided in a cryopreserved format. (Human Dermal Lymphatic Endothelial Cells (HDLEC) from the same donor are available on request.)The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are >95% CD31 positive and stain positive for von Willebrand factor. In addition, they are >90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.Blood Endothelial Cells have a key function in physiological processes like vessel tonus, capillary permeability, blood coagulation, fibrolysis, and angiogenesis. They are also useful for disease studies of atherosclerosis, tumor genesis, and thrombosis.

Calidad

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested forthe absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertencia

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rutina de subcultivo

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Otras notas

Recommended Plating Density: 10000 - 20000 cells per cm²Passage After Thawing: P2Tested Markers: Podoplanin negative, CD31 positiveGuaranteed population doublings: >15

Productos recomendados

Recommended Primary Cell Culture Media:Link

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable