COLLH-RO

Collagenase H

from Clostridium histolyticum

• Número CE: 3.4.24.3 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• origen biológico: Clostridium histolyticum
• Nivel de calidad: 100
• esterilidad: non-sterile
• Formulario: lyophilized
• collagenase activity: 0.15 U/mg lyophilizate
• envase: pkg of 100 mg (11074032001); pkg of 2.5 g (11087789001); pkg of 500 mg (11074059001)
• fabricante / nombre comercial: Roche
• concentración: ~1 mg/mL (Isolation of rat hepatocytes); ~2 mg/mL (Isolation of adipocytes)
• técnicas: tissue processing: suitable
• color: brown

Descripción

Descripción general

Collagenase H is an enzyme mixture prepared from Clostridium histolyticum cultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization.

Especificidad

Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins.

Aplicación

Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Collagenase H is well suited for the isolation of hepatocytes from rat liver by the collagenase perfusion method. This enzyme preparation is also suitable for the preparation of other types of cells such as endothelial cells from large vessels, and for the isolation of adipocytes from epididymal fat pads of rats.

Forma física

Lyophilizate, nonsterile

Nota de preparación

Enzyme activity:
Collagenase activity: >0.15U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate).
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase H has a balanced ratio of enzyme activities, and is function tested for the isolation of rat hepatocytes (perfusion method).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Activator: Ca²⁺
Working concentration: 0.5 to 2.5mg/ml,
Approximately 1 mg/ml for the isolation of rat hepatocytes and 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution should be stored at -15 to -25°C for short term storage, -60°C or as described below for long-term storage.

Reconstitución

Reconstitution in any balanced salt solution (e.g., HBSS)

Almacenamiento y estabilidad

Keep container tightly closed in a dry and well-ventilated place.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Pictogramas: GHS07,GHS08
• Palabra de señalización: Danger
• Frases de peligro: H315 - H319 - H334 - H335
• Consejos de prudencia: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Clasificaciones de peligro: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash