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Key Documents

SCT057

Sigma-Aldrich

BioTracker ERthermAC Temperature-Sensitive Live Cell Dye

Sinónimos:

Live cell imaging probe

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About This Item

técnicas

cell based assay: suitable

Descripción general

Brown adipocyte tissue (BAT) has been shown to be present in human newborns, small infants, as well as adults. Stimulation of BAT energy expenditure is considered a potential new drug target for obesity and diabetes treatments, and there is increasing interest in the discovery of novel mechanisms and substances for this purpose. However, to facilitate the development of breakthrough therapies, it is essential that techniques to measure the signature function of BAT are readily available.

The BioTracker ERthermAC thermosensitive live cell dye is a novel BODIPY-based dye that is capable of imaging heat production in individual brown adipocytes. The dye′s features such as small size, excellent cellular uptake, low cytotoxicity and robust photostability make it very biocompatible. Data obtained using this dye is comparable with that obtained by conventional methods measuring mitochondrial depolarization, oxygen consumption, and extracellular acidification rates, and with thermal power measurements obtained using the calScreener.

Spectral Properties:
  • Absorption: 565 nm
  • Emissions: 590 nm (Orange/Red) in polarized mitochondria
  • Emissions: 527 nm (Green) in depolarized mitochondria


References:
Kriszt R et al. Optical visualization of thermogenesis in stimulated single-cell brown adipocytes. Sci Rep. 2017 May 3;7(1):1383.

Aplicación

Application: Live cell fluorescent imaging
Live cell imaging thermosensitive dye to visualize heat production in brown adipocytes.

Calidad

Purity: ≥98% confirmed by HNMR, LC-MS and HPLC and elemental analysis

Molar Mass: 541.28 g/mol

Otras notas

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Información legal

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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