MABT338

Anti-phospho-cytokeratin-8 (K8) (Ser431) Antibody, clone 5B3

clone 5B3, from mouse

• Sinónimos: Keratin, type II cytoskeletal 8, CK-8, Keratin-8, K8, Type-II keratin Kb80
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: mouse
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 5B3, monoclonal
• reactividad de especies: human, mouse
• envase: antibody small pack of 25 μg
• técnicas: immunofluorescence: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001243222
• Nº de acceso UniProt: P05787
• modificación del objetivo postraduccional: phosphorylation (pSer431)
• Información sobre el gen: human ... KRT8(3856) ; mouse ... Krt8(16691)

Descripción

Descripción general

Keratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8 Keratin-8, K8, Type-II keratin Kb80) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human. Cytokeratin-8 is a type II, neutral to basic, protein of the intermediate filament family that together with Cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73. Serine 23 is shown to be highly conserved in all type II keratins. Phosphorylation at Serine 431 is reported to increase during mitosis and upon treatment with epidermal growth factor. Cytokeratin-8 can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation. Mutations in KRT8 gene have been linked to liver cirrhosis that is characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Two isoforms of Cytokeratin-8 have been described that are produced by alternative splicing.

Especificidad

Clone 5B3 specifically detects cytokeratin-8 phosphorylated on serine 431.

Inmunógeno

Cytokeratin-8/18 purified from okadaic acid-treated HT29 cells.

Aplicación

Anti-phospho-cytokeratin-8 (K8) (Ser431), clone 5B3, Cat. No. MABT338, is a mouse monoclonal antibody that detects type II Cytokeratin-8 phopshorylated on serine 431 and has been tested for use in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Immunofluorescence applications (Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).

Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Western Blotting applications (Yoon, K.H., et. al. (2001). J Cell Biol. 153(3):503-16; Ridge, K.M., et. al. (2005). J Biol Chem. 280(34):30400-5; Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).

Calidad

Evaluated by Western Blotting in lysates from HeLa cells treated with Calyculin A/Okadaic Acid.

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho-cytokeratin-8 (K8) (Ser431) in lysate from HeLa cells treated with Calyculin A/Okadaic Acid.

Descripción de destino

~53 kDa observed; 53.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Otras notas

Concentration: Please refer to lot specific datasheet.