MABN43

Anti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4

clone 4E4, from mouse

• Sinónimos: Apolipoprotein E, Apo-E, ApoE4, Apolipoprotein E4
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: 4E4, monoclonal
• application: IHC; IP; WB
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 2

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 4E4, monoclonal
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_000032
• Nº de acceso UniProt: P02649
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... APOE(348)

Descripción

Descripción general

Apolipoprotein is a polymorphic plasma protein that exists as three isoforms: ApoE4, ApoE3, and ApoE2, which differ in the combination of cysteine and arginine residues located at position 122 and 158. The 35 kDa ApoE4 isoform contains arginine at both locations, and is produced in the liver and brain, although it is widely expressed in other tissues, such as the lung, spleen, and ovary. Like other isoforms, ApoE4 is a ligand for low density lipoprotein receptors (LDL) and plays a critical role in the transport and metabolism of lipids. In addition to its role in maintaining cardiovascular function, ApoE4 is also critically involved in neuronal plasticity, synaptogenesis, aging, and neurodegenerative disorders. Mice deficient in ApoE4 show age-dependent abnormalities in synaptic structure and impaired long term potentiation. Additionally, ApoE4 has a well-documented role in the pathology of Alzheimer’s disease.

Especificidad

This antibody recognizes ApoE4 but not ApoE3 or ApoE2.

Inmunógeno

KLH-conjugated linear peptide corresponding to human ApoE4.

Aplicación

Immunohistochemistry Analysis: A 1:2,000-4,000 dilution from a representative lot detected ApoE4 in human Alzheimer′s diseased brain and human cerebellum tissues.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect ApoE4 in CHO cells secreting ApoE4. (Image courtesy of Dr. Egon Ogris, Medical University of Vienna)

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Anti--Apolipoprotein E4 antibody is validated for use in IP, WB, IH for the detection of ApoE4.

Calidad

Evaluated by Western Blot in recombinant ApoE4 protein.

Western Blot Analysis: A 1:100,000 dilution detected ApoE4 in 10 µg of recombinant ApoE4 protein.

Descripción de destino

~35 kDa observed

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Recombinant Apolipoprotein E protein

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable