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Key Documents

MABF2778

Sigma-Aldrich

Anti-IRF-3 Antibody, clone AR-1

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About This Item

Código UNSPSC:
12352203

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

AR-1, monoclonal

mol peso

calculated mol wt 47.22 kDa
observed mol wt ~51 kDa

reactividad de especies

rhesus macaque, human

envase

antibody small pack of 100 μL

técnicas

ELISA: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Descripción general

Interferon regulatory factor 3 (UniProt: Q14653; also known as IRF-3) is encoded by the IRF3 gene (Gene ID: 3661) in human. IRF-3 is a transcription factor that is constitutively expressed in a variety of tissues and plays a role in intracellular immune response against DNA and RNA viruses. It regulates the transcription of type I interferon genes (IFN-a and IFN-b) and interferon-stimulated genes by binding to an interferon-stimulated response element in their promoters. IRF-3 contains a DNA-binding domain (aa 5-111), nuclear export signal (aa 139-149), IRF-interacting domain, and a C-terminal serine-rich region that contains several phosphorylation sites. Although some of these serines are shown to be phosphorylated in the resting state, further phosphorylation of specific residues is required for its activation and nuclear translocation. In uninfected cells, IRF-3 is present in the cytoplasm, however, following infection, it undergoes phosphorylation on serine 385, serine 386, threonine 390, and serine 396. These phosphorylation events induce a conformational change that leads to its dimerization and nuclear localization where it associates with CREB binding protein to form dsRNA activated factor 1 that subsequently leads to the activation of type I interferons and ISG genes. Phosphorylation and subsequent activation of IRF-3 shown to be inhibited by vaccinia virus protein E3. Mutations in IRF3 gene have been linked to infection-induced, Herpes-specific acute encephalopathy that is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Clone AR-1 recognizes an epitope within the DNA-binding domain of IRF-3. It can detect resting IRF-3 as well as the activated form. (Ref.: Rustagi, A., et al. (2013). Methods. 59(2); 225-232; Honda, K., et al. (2006). Immunity. 25(3); 349-360).

Especificidad

Clone AR1 is a mouse monoclonal antibody that detects Interferon regulatory factor 3 (IRF-3). It targets an epitope within the DNA-binding domain from the N-terminal half.

Inmunógeno

His-tagged full-length human recombinant human Interferon regulatory factor 3 (IRF-3).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IRF-3 in A549 cell lysate.

Tested Applications

ELISA Analysis: A representative lot detected IRF-3 in ELISA applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Flow Cytometry Analysis: A representative lot detected IRF-3 in Flow Cytometry applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Western Blotting Analysis: A representative lot detected IRF-3 in Western Blotting applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Immunocytochemistry Analysis: A representative lot detected IRF-3 in Immunocytochemistry applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IRF-3, clone AR-1, Cat. No. MABF2778, is a mouse monoclonal antibody that detects Interferon regulatory factor 3 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1


Certificados de análisis (COA)

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