MABE1078

Anti-TAF10/TAFII30 Antibody, clone 23TA-1H8

ascites fluid, clone 23TA-1H8, from mouse

• Sinónimos: Transcription initiation factor TFIID subunit 10, STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: ascites fluid
• tipo de anticuerpo: primary antibodies
• clon: 23TA-1H8, monoclonal
• reactividad de especies: human
• no debe reaccionar con: mouse
• técnicas: ChIP: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_006275
• Nº de acceso UniProt: Q12962
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... TAF10(6881)

Descripción

Descripción general

Transcription initiation factor TFIID subunit 10 (UniProt Q12962; also known as STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit) is encoded by the TAF10 (also known as TAF2A, TAF2H, TAFII30) gene (Gene ID 6881) in human.Transcription initiation factor TFIID subunit 10 (UniProt Q12962; also known as STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit) is encoded by the TAF10 (also known as TAF2A, TAF2H, TAFII30) gene (Gene ID 6881) in human. TAF10 (TAFII30) is a subunit of both the general transcription factor (GTF) TFIID and the coactivator SAGA (Spt-Ada-Gcn5-acetyltransferase) histone acetyltransferase (HAT) complexes involved in the initiation of RNA polymerase II (Pol II) transcription in eukaryotes. TFIID complex is composed of the TATA-binding protein (TBP) and thirteen TBP-associated factors (TAFs) that allow its recognition of not only TATA box-containing, but also TATA-less promoters for site-specific assembly of preinitiation complex (PIC). TAF10-knockout studies in mice indicate that TAF10 plays an important role during embryonic development and homeostasis in a tissue-dependent manner. TAF10 ablation is reported to result in severe anemia and lethality at embryonic stage E13.5 due to impaired erythropoiesis. In addition, TAF10 is shown to not only interact with the transcription factor GATA1, but also regulate its transcription as evidenced by its occupancy at GATA1 genomic locus in human fetal erythroid cells.

Especificidad

Clone 23TA-1H8 (a.k.a. 1H8, 23TA 1H8, 23TA1H8) recognizes an N-terminal epitope present in human (UniProt Q12962), but not mouse (UniProt Q8K0H5), TAF10 (TAFII30) sequence.

Inmunógeno

Epitope: Near-N-terminus.

Ovalbumin-conjugated linear peptide corresponding to the N-terminal sequence of human TAF10/TAFII30.

Aplicación

Anti-TAF10 Antibody, clone 23TA-1H8 is an antibody against TAFII30 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP).

Chromatin Immunoprecipitation Analysis: A representative lot detected TAF10 occupancy at the GATA1 promoter region by ChIP using chromatin preparations from human fetal liver (hFL) and adult human peripheral blood (hPB) derived erythroid progenitor (HEP) cells. A greater GATA1 promoter enrichment was seen in hFL than in hPB ChIP (Papadopoulos, P., et al. (2015). Mol. Cell. Biol. 35(12):2103-2118).
Western Blotting Analysis: A representative lot detected TAF10 (TAFII30) in HeLa nuclear extracts as well as in SPT20-containing SAGA immune complex, but not in ADA2a-containing ATAC immune complex (Nagy, Z, et al. (2010). Cell. Mol. Life Sci. 67(4):611-628).
Western Blotting Analysis: Representative lots detected the presence of TAF10 (TAFII30) in TAFII30-containing complexes immunoprecipitated from HeLa nuclear extracts (Nagy, Z., et al. (2009). Mol .Cell. Biol. 29(6):1649-1660; Wieczorek, E., et al. (1998). Nature. 393(6681):187-191).
Immunoprecipitation Analysis: Representative lots immunoprecipitated TAF10- (TAFII30-) containing complexes from HeLa nuclear extracts (Nagy, Z., et al. (2009). Mol .Cell. Biol. 29(6):1649-1660; Wieczorek, E., et al. (1998). Nature. 393(6681):187-191).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Calidad

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TAF10/TAFII30 in 10 µg of A431 cell lysate.

Descripción de destino

~30 kDa observed. 21.71 kDa calculated.

Forma física

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable