ABS2129

Anti-IP3R 1

from rabbit

• Sinónimos: Inositol 1, 4, 5-trisphosphate receptor type I, IP3 receptor isoform 1, IP-3-R1, InsP3R1, Type 1 inositol 1, 4, 5-trisphosphate receptor, Type 1 InsP3 receptor
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: human, rat, bovine, mouse, chicken
• técnicas: immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_001257526
• Nº de acceso UniProt: P29994
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ITPR1(3708) ; rat ... Itpr1(25262)

Descripción

Descripción general

Inositol 1,4,5-trisphosphate receptor type 1 (UniProt: P29994; also known as IP3 receptor isoform 1, IP-3-R, IP3R 1, InsP3R1, Type 1 inositol 1,4,5-trisphosphate receptor, Type 1 InsP3 receptor) is encoded by the Itpr1 (also known as Insp3r) gene (Gene ID: 25262) in rat. InsP3R1 is a multi-pass membrane protein located on endoplasmic reticulum (ER) membrane. Its highest levels are found in neurons, with very high expression in the Purkinje cells of the cerebellum. InsP3R1 contains a calcium channel in its C-terminal extremity. It has a large N-terminal cytoplasmic region that has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. InsP3R1 serves as an intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.It is involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1. It also plays a role in ER stress-induced apoptosis wherein cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways. InsP3R1 is phosphorylated by cAMP-dependent protein kinase (PKA), which increases the interaction with inositol 1,4,5-trisphosphate and reduces the interaction with AHCYL1. This prevents the ligand-induced opening of the calcium channels. Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium-binding protein which then inhibits the receptor. Reduced expression of InsP3R1 has been reported in cisplatin-resistant cancer cells and overexpression of InsP3R1 in resistant cells induces apoptosis and increases sensitivity to cisplatin. (Ref.: Tsunoda T., et al. (2005). Oncogene. 24(8):1396-402; Wang, Y., et al. (2001). Cir. Res. 88(2): 202-209).

Especificidad

This polyclonal antibody specifically react with IP3 Receptor 1 in multiple species, but does not recognize IP3R2 and IP3R3. It targets an epitope within 19 amino acids from the C-terminal region.

Inmunógeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of rat Inositol 1,4,5-trisphosphate receptor type 1 (IP3R1).

Aplicación

Detect Inositol 1,4,5-trisphosphate receptor type 1 using this rabbit polyclonal Anti-IP3R1, Cat. No. ABS2129 that has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. ,

Research Category
Signaling

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IP3R1 in 10 µg of rat and mouse brain microsomal preparation.

Western Blotting Analysis: A representative lot detected IP3R1 in bovine salivary gland, DT40 3KO cells stably expressing IP3R1 (Chandrasekhar, R., et. al. (2016). J Biol Chem. 291(10):4846-60).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected IP3R1 in human brain and rat brain tissues.

Immunoprecipitation Analysis: A representative lot detected IP3R1 in bovine salivary gland (Chandrasekhar, R., et. al. (2016). J Biol Chem. 291(10):4846-60).

Calidad

Evaluated by Western Blotting in rat brain microsomal preparation.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IP3R1 in 10 µg of rat brain microsomal preparation.

Descripción de destino

~300 kDa observed; 313.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 1% BSA and 0.02% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable