ABS1007

Anti-phospho-Caveolin-1 (Ser37)

from rabbit, purified by affinity chromatography

• Sinónimos: CAV-1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• técnicas: inhibition assay: suitable (peptide); western blot: suitable
• Nº de acceso NCBI: NP_001744.2
• Nº de acceso UniProt: Q03135
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: phosphorylation (pSer37)
• Información sobre el gen: human ... CAV1(857)

Descripción

Descripción general

Caveolin-1 (UniProt: Q03135) is encoded by the CAV1 (also known as CAV) gene (Gene ID: 857) in human. Three caveolin genes are expressed in mammals and are designated as caveolin-1, -2, and -3. Most mammalian tissues express at least one of these isoforms. Caveolin-1 is expressed in skeletal muscle, liver, stomach, lung, brain, kidney and heart. Caveolin-1 and -2 are usually co-expressed and assemble into hetero-oligomers in the endoplasmic reticulum and Golgi apparatus. Two isoforms of caveolin-1 have been reported that are produced by alternative initiation. It has two cytoplasmic domains (aa 2-104 and 126-178) and an intramembrane domain (aa 105-125). The primary structure of caveolin-1 contains two serine residues, both residing in a phosphorylation consensus sequence and flanked by a regulatory scaffolding domain. Serine 37 site represents a PKC consensus site, while Serine 88 is believed to be phosphorylated by CK II alpha. Caveolin-1 acts as a scaffolding protein within caveolar membranes and is shown to interact directly with G-protein alpha subunits to functionally regulate their activity. It is also involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. It can bind to DPP4 and induce T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Mutations in CAV1 gene are known to cause Congenital generalized lipodystrophy 3 that is characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, and early onset of diabetes. Some mutations have also been linked to primary pulmonary hypertension with plexiform lesions of proliferating endothelial cells in pulmonary arterioles leading to elevated pulmonary arterial pression, right ventricular failure, and death. (Ref.: Liu, P., et al. (2002). J. Biol. Chem. 277 (44); 41295-41298; Garver, WS., et al. (1999). Biocheim. Biophys. Acta 143 (1453); 193-206).

Especificidad

This rabbit polyclonal antibody specifically detects Caveolin-1 phosphorylated on Serine 37.

Inmunógeno

KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phospho Serine 37 from the N-terminal region of human Caveolin-1. The immunogen sequence is conserved in mouse and rat.

Aplicación

Anti-phospho-Caveolin-1 (Ser37), Cat. No. ABS1007, is a rabbit polyclonal antibody that detects Caveolin-1 phosphorylated on Serine 37 and has been tested for use in Peptide Inhibition Assay and Western Blotting.

Peptide Inhibition Analysis: A Caveolin-1 phosphopeptide (Ser37) blocked the detection of phospho-Caveolin-1 (Ser37) in lysates of A431 cells treated with Sodium Pervanadate.

Research Category
Signaling

Calidad

Evaluated by Western Blotting in A431 cells treated with sodium pervanadate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-Caveolin-1 (Ser37) in A431 cells treated with sodium pervanadate (5 mM, 30 minutes).

Descripción de destino

~21 kDa observed; 20.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash