ABE446

Anti-Monomethyl Histone H1 (Lys26) Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Histone H1.3, Histone H1c, Histone H1s-2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• técnicas: dot blot: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_005311
• Nº de acceso UniProt: P16402
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: monomethylation (Lys26)
• Información sobre el gen: human ... HIST1H1D(3007)

Descripción

Descripción general

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes, and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.

Especificidad

This antibody recognizes the N-terminus of Monomethyl Histone H1 (Lys26) methylated at Lys26.

Inmunógeno

KLH-conjugated linear peptide corresponding to the N-terminus of human Monomethyl Histone H1 (Lys26).

Aplicación

Detect Histone H1 using this rabbit polyclonal antibody, Anti-Monomethyl Histone H1 (Lys26) Antibody validated for use in western blotting, Dot Blot & IP.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Monomethyl Histone H1 (Lys26) in 500 µg of HeLa acid extract.
Dot Blot Specificity Analysis: This antibody detected Monomethyl Histone H1 (Lys26) in in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and in an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Calidad

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Monomethyl Histone H1 (Lys26) in 10 µg of HeLa acid extract.

Descripción de destino

~32 kDa observed

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1