ABC502

Anti-PAR-4 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: PAR-4, PRKC apoptosis WT1 regulator protein, Prostate apoptosis response 4 protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), canine (based on 100% sequence homology), horse (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_002574
• Nº de acceso UniProt: Q96IZ0
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PAWR(5074)

Descripción

Descripción general

PAR-4 is a pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells and sensitizing the cells to diverse apoptotic stimuli causing tumor regression. PAR-4 also seems to be a transcriptional repressor and is involved in regulating APP expression and the cleavage activity of BACE1. PAR-4 interacts with several proteins including WT1, but also various kinases, and structural proteins like Actin, BACE1, SPSB1, SPSB2, and SPSB4. PAR4 is localized in the cytoplasm in non-apoptotic cells but is nuclear localized in cancer and apoptotic cells. PAR-4 is widely expressed and elevated in neurodegenerative diseases such as ALS, Alzheimer, Parkinson and Huntington diseases as well as stroke. PAR-4 is down regulated in cancers.

Inmunógeno

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human PAR-4.

Aplicación

Anti-PAR-4 Antibody is an antibody against PAR-4 for use in Western Blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Calidad

Evaluated by Western Blotting in Y-79 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PAR-4 in 10 µg of Y-79 cell lysate.

Descripción de destino

~45 kDa observed. The calculated molecular weight is 36 kDa, however PAR-4 has been shown as a ~45 kDa band in some western blots (Masters, S.L., et al. (2006). Nature Structural & Mole. Biol. 13(1):77-84).

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable