616371

Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free

recombinant, expressed in Nicotiana, heat and acid-stable competitive, reversible inhibitor of serine proteases

• Sinónimos: Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free, Pancreatic Trypsin Inhibitor, Trypsin-Kallikrein Inhibitor, Kallikrein-Trypsin Inactivator, Antikrein, Basic Pancreatic Trypsin Inhibitor
• Fórmula empírica (notación de Hill): C₂₈₄H₄₃₂N₈₄O₇₉S₇
• Número de CAS: 9087-70-1
• Peso molecular: 6511.44
• Número MDL: MFCD00130541
• Código UNSPSC: 12352202
• Número índice de la CE: 2329949
• NACRES: NA.77

Propiedades

• recombinante: expressed in Nicotiana
• Nivel de calidad: 200
• Análisis: ≥98% (SDS-PAGE)
• formulario: powder
• actividad específica: ≥5.0 units/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• color: white to off-white
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Recombinant, bovine aprotinin supplied without animal-derived components. Aprotinin is a competitive, reversible inhibitor of proteolytic and esterolytic activity. A relatively heat- and acid-stable serine protease inhibitor. Forms a tight complex, blocking the active site of the enzyme. Effective at concentrations equimolar with protease. Inhibits several proteases, including coagulation factors in the prephase of blood clotting, tissue and leukocytic proteinases, chymotrypsin, trypsin (K_d = 5 x 10^-14 M), plasmin (K_d = 2.3 x 10^-10 M), and kallikrein (K_d = 1 x 10^-7 M). Proteases not inhibited by aprotinin include Factor Xa, thrombin, pepsin, papain, and carboxypeptidases A and B. Useful for protein purification and for extending the life of cells in culture by preventing proteolytic damage.

Acciones bioquímicas o fisiológicas

Cell permeable: no

Kd = 5 x 10-14 M, 2.3 x 10-10 M, 1 x 10-7 M against trypsin, plasmin, and kallikrein, respectively

Primary Target
chymotrypsin

Product does not compete with ATP.

Reversible: yes

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One TIU (Trypsin Inhibitory Unit) will decrease the activity of two trypsin units by 50% where one trypsin unit will hydrolyze 1.0 µmole of Nα-benzoyl-L-Arginine-p-Nitroanilide (L-BAPNA) per minute at pH 7.8 at 25°C.

Forma física

Contains no animal-derived components.

Otras notas

Deutscher, M.P., 1990. Methods Enzymol.182, 83.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable