521225

PDGF, BB Homodimer, Human, Recombinant, E. coli

• Sinónimos: rhPDGF-BB
• Código UNSPSC: 12352202

Propiedades

• Análisis: ≥95% (SDS-PAGE)
• Nivel de calidad: 100
• formulario: lyophilized
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• impurezas: ≤1 ng/μg Endotoxin (ng/μg PDGF-BB)
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Recombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal and mononuclear cells. Has the same amino acid sequence as mature human PDGF-B. Rapidly activates protein kinase D in vascular smooth muscle cells.

Recombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal, and mononuclear cells. PDGF represents one of the major mitogens present in platelets. It exerts its effects by binding to specific high-affinity dimeric receptor tyrosine kinases. Material has the same amino acid sequence as mature human PDGF-B. Two distinct PDGF receptors have been identified: the PDGFα receptor, which binds to all three forms of PDGF, and the PDGFβ receptor, which can bind only PDGF-BB and PDGF-AB.

Acciones bioquímicas o fisiológicas

ED₅₀ = 1-5 ng/ml as determined by the dose dependent proliferation of BALB/c 3T3 cells

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from a sterile-filtered solution, carrier free.

Reconstitución

Reconstitute to a concentration of ≥100 µg/ml 100 mM acetic acid, 0.1% BSA. Further dilute with low endotoxin medium or buffered solution containing FBS or tissue culture grade BSA just prior to use.

Otras notas

Abedi, H., et al. 1998. FEBS Lett. 427, 209.
Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
Westermark, B., and Heldin, C.-H. 1991. Cancer Res.51, 5087.
Raines, E.W., et al. 1985. Methods Enzymol.109, 749.
Johnson, A., et al. 1984. EMBO J.3, 921.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable