239807

Cyclopamine-KAAD

InSolution, ≥70%, inhibits Hedgehog (Hh) signaling

• Sinónimos: 3- Keto-N-( aminoethyl- aminocaproyl- dihydrocinnamoyl)cyclopamine, 3-Keto-N-(aminoethyl-aminocaproyl-dihydrocinnamoyl)cyclopamine, InSolution Cyclopamine-KAAD
• Código UNSPSC: 12352116

Propiedades

• Nivel de calidad: 100
• Análisis: ≥70% (sum of two isomers, HPLC)
• formulario: liquid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

A cell-permeable potent analog of Cyclopamine (>Cat. No. 239803) that specifically inhibits Hedgehog (Hh) signaling with similar or lower toxicity (IC₅₀ = 20 nM in Shh-LIGHT2 assay; 50 nM in p2^Ptch-/-cells; 500 nM in SmoA1-LIGHT cells). Binds to SmoA1 and promotes its exit from the endoplasmic reticulum. Suppresses both ShhNp-induced pathway activity and SmoA1-induced reporter activity. Reported to cause regression of murine tumor allografts in vivo and induce rapid cell death in human medulloblastoma. Shown to sensitize human glioma cells to TRAIL-induced apoptosis. The solid form of this compound (Cat. No. 239804) is also available.

Acciones bioquímicas o fisiológicas

Cell permeable: yes

Primary Target
SmoA1

Envase

Packaged under inert gas

Advertencia

Toxicity: Irritant (B)

Forma física

A 1 mM (50 µg/72 µL) solution of Cyclopamine-KAAD (Cat. No. 239804) in DMSO.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C.

Otras notas

Siegelin, M.D. et al. 2009. Neurobiol. Dis.34, 259.
Watkins, D.N., et al. 2003. Nature422, 313.
Berman, D.M., et al. 2002. Science297, 1559.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.
Chen, J.K., et al. 2002. Genes Dev.16, 2743.
Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.
Taipale, J., et al. 2000. Nature406, 1005.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): 188.6 °F - (refers to pure substance)
• Punto de inflamabilidad (°C): 87 °C - (refers to pure substance)