23-026-M

SCF-TrCP1 Protein Complex, Active, 10 µg

Active complex of four (4) recombinant human enzymes: full length Skp1, full length Cul1, full length Rbx1, & βTrCP1 (amino acids 53-end). For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

• Sinónimos: SCF-β-TrCP, SCF ^FWD1, SCF-bTrCP1
• Código UNSPSC: 12352200
• eCl@ss: 32160405
• NACRES: NA.41

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• fabricante / nombre comercial: Upstate^®
• técnicas: activity assay: suitable
• Nº de acceso NCBI: NM_003592; NM_014248; NM_033637; NM_170679

Descripción

Descripción general

N-terminal GST-tagged, recombinant human Smurf1 full length, expressed in E coli.

The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCF^βTrCP1 mediates the ubiquitination and subsequent proteasomal degradation of a variety of proteins involved in various cellular processes, these include IκBα, an inhibitor of the transcription factor NFκB and β-catenin which is involved in the Wnt signalling pathway. A number of cancers, including those in breast, colon and pancreatic tissue have been associated with the deregulation of SCF^βTrCP1

Aplicación

Ubiquitination Cascade Comoponent: E3

Forma física

Purified using glutathione sepharose.

Otras notas

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H317
• Consejos de prudencia: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Clasificaciones de peligro: Skin Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°C): Not applicable