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Merck
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Key Documents

17-300

Sigma-Aldrich

Ral Activation Assay Kit

Non-radioactive Ral Activation Assay Kit can be used to precipitate Ral-A-GTP from cell lysates & detection by a Ral-A specific antibody.

Sinónimos:

Activation Assay Kit, Ral Assay Kit

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About This Item

Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Nivel de calidad

fabricante / nombre comercial

Upstate®

técnicas

activity assay: suitable
affinity binding assay: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

dry ice

Información sobre el gen

human ... RALA(5898)

Envase

Kit capacity: 30 assays

Componentes

Ral Activation Assay Buffer, 5X (Cat.# 20-196)

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Anti-Ral-A

Ral Assay Reagent (Ral BP1, agarose) (Cat.# 14-415)

Calidad

Routinely evaluated by affinity precipitation/immunoblot analysis. Ral-A-GTP was precipitated from EGF-stimulated Cos-7 cell lysates. The precipitated Ral-A-GTP was detected by Western immunoblot analysis using anti-Ral A.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogramas

Corrosion

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Aquatic Chronic 3 - Eye Dam. 1

Código de clase de almacenamiento

10 - Combustible liquids


Certificados de análisis (COA)

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2'-Methylseleno-modified oligoribonucleotides for X-ray crystallography synthesized by the ACE RNA solid-phase approach.
Barbara Puffer,Holger Moroder,Michaela Aigner,Ronald Micura
Nucleic Acids Research null
Nicolas Personnic et al.
The FEBS journal, 281(13), 2977-2989 (2014-05-13)
Ral proteins are small GTPases that play critical roles in normal physiology and in oncogenesis. There is little information on the GTPase-activating proteins (GAPs) that downregulate their activity. Here, we provide evidence that the noncatalytic β subunit of RalGAPα1/2 β
Farnaz Yamoutpour et al.
Molecular cancer therapeutics, 7(11), 3586-3597 (2008-11-13)
Epidermal growth factor receptor variant III (EGFRvIII) is a constitutively active mutant form of EGFR that is expressed in 40% to 50% of gliomas and several other malignancies. Here, we describe the therapeutic effects of silencing EGFRvIII on glioma cell
J de Rooij et al.
Oncogene, 14(5), 623-625 (1997-02-06)
Ras is a small GTPase that cycles between an inactive GDP-bound and an active GTP-bound form. A large variety of ligands that stimulate cell surface receptors induce the activation of Ras. Thus far, this activation could only be measured by
M Foschi et al.
The EMBO journal, 16(21), 6439-6451 (1997-11-14)
Endothelin-1 (ET-1) induces cell proliferation and differentiation through multiple G-protein-linked signaling systems, including p21ras activation. Whereas p21ras activation and desensitization by receptor tyrosine kinases have been extensively investigated, the kinetics of p21ras activation induced by engagement of G-protein-coupled receptors remains

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