1.01374

Brilliant green (hydrogen sulfate) (C.I. 42040)

for microscopy Certistain^®

• Sinónimos: Brilliant green (hydrogen sulfate) (C.I. 42040), Diamond green G, Solid green, Ethyl green
• Fórmula empírica (notación de Hill): C₂₇H₃₄N₂O₄S
• Número de CAS: 633-03-4
• Peso molecular: 482.63
• Número MDL: MFCD00011880
• Código UNSPSC: 41116130
• Número índice de la CE: 211-190-1

Propiedades

• Nivel de calidad: 400
• formulario: solid
• potencia: 300-400 mg/kg LD₅₀, oral (Rat)
• composición: Dye, ≥95% spectrophotometric assay
• pH: 2.5 (25 °C, 10 g/L in H₂O)
• mp: 210 °C (decomposition)
• solubilidad: 100 g/L
• densidad aparente: 500‑600 kg/m³
• εmax: 2210-2350 at 628-632 nm in 50% ethanol at 0.003 g/L
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: 2-30°C
• InChI: 1S/C27H33N2.H2O4S/c1-5-28(6-2)25-18-14-23(15-19-25)27(22-12-10-9-11-13-22)24-16-20-26(21-17-24)29(7-3)8-4;1-5(2,3)4/h9-21H,5-8H2,1-4H3;(H2,1,2,3,4)/q+1;/p-1
• Clave InChI: NNBFNNNWANBMTI-UHFFFAOYSA-M

Descripción

Descripción general

Brilliant green (hydrogen sulfate) (C.I. 42040) for microscopy Certistain^®, is a dry staining dye that is used to prepare a staining solution makes target structures in bacteriological specimen materials evaluable for diagnostic purposes. In the area of bacteriology, among others the Gram staining method, the Ziehl-Neelsen staining method, and the spore-staining method according to Rakette are used to yield a differentiated image of pathogens. This can be done either by using eosin Y as a counterstain or by modification according to Wirtz using safranine O solution. By this method, spores are always stained green and the cell elements always red.
As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Nota de análisis

Dye content (spectrophotometrically): ≥ 95 %
Identity
- Hydrogen sulfate (as sulfur):
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 628 - 632 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2210 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Información legal

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H302 - H319
• Consejos de prudencia: P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P337 + P313
• Clasificaciones de peligro: Acute Tox. 4 Oral - Eye Irrit. 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°C): Not applicable