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Merck
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Documentos clave

09-774

Sigma-Aldrich

Anti-EED Antibody

from rabbit

Sinónimos:

embryonic ectoderm development

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

rabbit

Nivel de calidad

100

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

reactividad de especies

human, mouse

reactividad de especies (predicha por homología)

dog, chicken, bovine, opossum, rat

técnicas

western blot: suitable

Nº de acceso NCBI

NP_003788

Nº de acceso UniProt

O75530

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1 of 4

Este artículo
05-1320-M03-19607-1562
Anti-EED Antibody from rabbit

09-774

Anti-EED Antibody

Anti-EED Antibody, clone AA19 ascites fluid, from mouse

05-1320-M

Anti-EED Antibody, clone AA19

RIPAb+ EED - RIP Validated Antibody and Primer Set purified antibody, from mouse

03-196

RIPAb+ EED - RIP Validated Antibody and Primer Set

Anti-EZH2 Antibody serum, Upstate®

07-1562

Anti-EZH2 Antibody

species reactivity

human, mouse

species reactivity

human, mouse

species reactivity

human, mouse

species reactivity

rat, mouse, human

antibody form

purified antibody

antibody form

ascites fluid

antibody form

purified antibody

antibody form

serum

clone

polyclonal

clone

monoclonal

clone

monoclonal

clone

polyclonal

UniProt accession no.

O75530

UniProt accession no.

O75530

UniProt accession no.

O75530

UniProt accession no.

Q15910

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

RIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: suitable

Descripción general

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.

Especificidad

This antibody recognizes EED, Mr ~ 62-70 kDa.

Inmunógeno

A synthetic peptide corresponding to a.a. 429-441 of human EED, conjugated to KLH.
Epitope: a.a. 429-441

Aplicación

Use Anti-EED Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EED also known as embryonic ectoderm development.

Calidad

Western Blot Analysis: 1 μg/mL of this lot detected EED on 10 μg of Jurkat cell lysate.

Descripción de destino

~53 kDa observed. Uniprot describes three isoforms at 51 kDa (isoform 1), 53 kDa (isoform 2), and 46 kDa (isoform 3).

Ligadura / enlace

Replaces: 09-727

Forma física

Format: Purified

Nota de análisis

Control
Jurkat Cell Lysate

3T3/A31 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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    Ji Young Oh et al.
    Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 46(5), 1749-1767 (2018-05-01)
    Glucose plays an important role in stem cell fate determination and behaviors. However, it is still not known how glucose contributes to the precise molecular mechanisms responsible for stem cell migration. Thus, we investigate the effect of glucose on the
    Epigenetic therapy with 3-deazaneplanocin A, an inhibitor of the histone methyltransferase EZH2, inhibits growth of non-small cell lung cancer cells.
    Junko Kikuchi,Taichi Takashina,Ichiro Kinoshita,Eiki Kikuchi,Yasushi Shimizu et al.
    Lung Cancer null
    Bin Shi et al.
    Cancer medicine, 8(14), 6383-6392 (2019-08-29)
    The aims of this study were to investigate the link between enhancer of zeste homolog 2 (EZH2) and histone deacetylase (HDAC) in preclinical studies and in human lung cancer tissue microarrays. Enhancer of zeste homolog 2 and HDAC1 mRNA expression
    Andrei Kuzmichev et al.
    Molecular cell, 14(2), 183-193 (2004-04-22)
    Human Enhancer of Zeste homolog (Ezh2) is a histone lysine methyltransferase (HKMT) associated with transcriptional repression. Ezh2 is present in several distinct complexes, one of which, PRC2, we characterized previously. Here we report an additional Ezh2 complex, PRC3. We show
    The central role of EED in the orchestration of polycomb group complexes.
    Cao, Q; Wang, X; Zhao, M; Yang, R; Malik, R; Qiao, Y; Poliakov, A; Yocum, AK; Li, Y; Chen et al.
    Nature Communications null
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