07-2113

Anti-phospho-Ribosomal Protein S6 Antibody (Ser240/Ser244)

from rabbit, purified by affinity chromatography

• Sinónimos: 40S ribosomal protein S6, Phosphoprotein NP33
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): bovine (based on 100% sequence homology), rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_001001
• Nº de acceso UniProt: P62753
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pSer240/pSer244)
• Información sobre el gen: human ... RPS6(6194)

Descripción

Descripción general

S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that are known to be phosphorylated by both p70S6 Kinase as well as RSK and Ser240/244 which is thought to only be phosphorylated by p70S6K.

Especificidad

This antibody recognizes Ribosomal Protein S6 phosphorylated at Ser240/Ser244.

Inmunógeno

Epitope: Phosphorylated Ser240/Ser244

KLH-conjugated linear peptide corresponding to human Ribosomal Protein S6 phosphorylated at Ser240/Ser244.

Aplicación

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Ribosomal Protein S6 in A431 cells and PMA treated A431 cells.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

This phospho-Ribosomal Protein S6 antibody is validated for use in WB & ICC for the detection of the phospho-Ribosomal Protein S6 protein.

Calidad

Evaluated by Western Blot in PMA treated and untreated A431 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected Ribosomal Protein S6 on 10 µg of PMA treated and untreated A431 cell lysates.

Descripción de destino

~32 kDa observed

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
PMA treated and untreated A431 cell lysates

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable