06-519-AF647

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™647 conjugate

from rabbit, ALEXA FLUOR^™ 647

• Sinónimos: Cyclic AMP-responsive element-binding protein 1 (pSer133), cAMP-responsive element-binding protein 1 (pSer133), CREB-1 (pSer133), CREB-A (pSer133), htCREB (pSer133), CREB-B (pSer119)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• conjugado: ALEXA FLUOR^™ 647
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse, rat, human
• reactividad de especies (predicha por homología): porcine (based on 100% sequence homology), guinea pig (based on 100% sequence homology), Xenopus (based on 100% sequence homology), canine (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology)
• técnicas: immunofluorescence: suitable
• isotipo: IgG1
• Nº de acceso NCBI: NP_604391
• Nº de acceso UniProt: P16220
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pSer133)
• Información sobre el gen: human ... CREB1(1385)

Descripción

Descripción general

Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.

Especificidad

Recognizes phosphorylated CREB-A (pSer133), htCREB (pSer133), and CREB-B (pSer119) spliced isoforms reported by (UniProt P16220). May also recognize phosphorylated CREM and ATF-1 that contain homologous sequence to that of the immungen peptide.

Inmunógeno

Epitope: pSer133.

KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB-A.

Aplicación

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™647 conjugate is an antibody against phospho-CREB for use in Immunofluorescence.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

The unconjugated antibody (Cat. No. 06-519) is shown to be suitable also for Electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry, and Western blotting applications.

Calidad

Evaluated by Immunofluorescence in rat brain tissue.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected phospho-CREB (Ser133) in rat brain tissue.

Descripción de destino

~43 kDa observed.

Forma física

Affinity purified

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Información legal

ALEXA FLUOR is a trademark of Life Technologies

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable