05-623-AF647

Anti-RNA polymerase II Antibody, clone CTD4H8, Alexa Fluor^™ 647 Conjugate

clone CTD4H8, from mouse, ALEXA FLUOR^™ 647

• Sinónimos: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: ALEXA FLUOR^™ 647
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: CTD4H8, monoclonal
• reactividad de especies: mouse, human
• reactividad de especies (predicha por homología): rat (based on 100% sequence homology), yeast (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable
• isotipo: IgG1
• Nº de acceso NCBI: NP_000928.1
• Nº de acceso UniProt: P24928
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... POLR2A(5430) ; mouse ... Polr2A(20020) ; rat ... Polr2A(363633)

Descripción

Descripción general

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Especificidad

This antibody recognizes Phospho- and non-phospho-RNA polymerase II.

Inmunógeno

Epitope: carboxyl-terminal domain of RNA polymerase II

KLH-conjugated, synthetic peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.

Aplicación

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor 647 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.

Calidad

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in HeLa cells.

Descripción de destino

~210-220 kDa observed

Forma física

Protein G Purified

Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Información legal

ALEXA FLUOR is a trademark of Life Technologies

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable